Dynamics of in vivo ASC speck formation

Dynamics of ASC speck formation during skin inflammatory responses in vivo

17 Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into 18 specks, large signalling platforms considered hallmarks of inflammasome activation. Because a 19 lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live 20 ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong 21 ASC express...

Zebrafish earns its stripes for in vivo ASC speck dynamics

Assembly of the ASC speck is critical for signaling by the inflammasome. In this issue, Kuri et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201703103) use live microscopy to track fluorescently tagged endogenous ASC in the zebrafish, describing the molecular domains driving ASC speck assembly and identifying a key role for macrophages in ASC speck removal in vivo.

Correction: Dynamics of in vivo ASC speck formation

Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation

Despite its clinical importance in infection and autoimmunity, the activation mechanisms of the NLRP1b inflammasome remain enigmatic. Here we show that deletion of the inflammasome adaptor ASC in BALB/c mice and in C57BL/6 macrophages expressing a functional NLRP1b prevents anthrax lethal toxin (LeTx)-induced caspase-1 autoproteolysis and speck formation. However, ASC(-/-) macrophages undergo n...

MiR-143 Induces Expression of AIM2 and ASC in Jurkat Cell Line

Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information ...